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Chromatography: AQA A‑Level Chemistry questions

Every AQA A-Level Chemistry exam question on 3.3.16 Chromatography, 2016–2025, with its mark scheme and examiner’s report.

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2,301 questions

  1. Q34

    June 2024 · Paper 3

    The whole paper ↗
    June 2024 Paper 3, question 34: The diagram shows a gas chromatogram for a sample containing four isomers with the molecular formula C6H12O2 The carrier gas is nitrogen and the stationary phas
  2. Q03.4

    June 2023 · Paper 3

    The whole paper ↗
    June 2023 Paper 3, question 03.4: Figure 3 shows the apparatus used for the TLC. Figure 3 There is a piece of the apparatus missing from Figure 3. This omission will result in an inaccurate chro
  3. Q03.5

    June 2023 · Paper 3

    The whole paper ↗
    June 2023 Paper 3, question 03.5: State why the amino acids separate on the TLC plate. [1 mark]
  4. Q03.6

    June 2023 · Paper 3

    The whole paper ↗
    June 2023 Paper 3, question 03.6: Name a suitable developing agent. State why the developing agent is needed. [2 marks] Name Why needed
  5. Q03.7

    June 2023 · Paper 3

    The whole paper ↗
    June 2023 Paper 3, question 03.7: Determine the Rf value for Tyr. [1 mark] Rf
  6. Q02.1

    November 2021 · Paper 3

    The whole paper ↗
    November 2021 Paper 3, question 02.1: A student uses thin-layer chromatography (TLC) to identify these amino acids. The student identifies two of the amino acids as alanine and serine. Use Figure 3
  7. Q02.2

    November 2021 · Paper 3

    The whole paper ↗
    November 2021 Paper 3, question 02.2: The amino acids cannot be seen as they move during the experiment. State how the amino acids can be made visible at the end of the experiment. [1 mark]
  8. Q02.3

    November 2021 · Paper 3

    The whole paper ↗
    November 2021 Paper 3, question 02.3: State why each amino acid has a different Rf value. [1 mark]
  9. Q09.2

    June 2019 · Paper 2

    The whole paper ↗
    June 2019 Paper 2, question 09.2: Suggest how the positions of the amino acids on the TLC plate were located. [1 mark]
  10. Q09.3

    June 2019 · Paper 2

    Mark scheme (PDF)
    The whole paper ↗
    June 2019 Paper 2, question 09.3: Deduce the minimum number of amino acids present in the original mixture. [1 mark]
  11. Q09.4

    June 2019 · Paper 2

    The whole paper ↗
    June 2019 Paper 2, question 09.4: Suggest why it was necessary to use two different solvents. [1 mark]
  12. Q03.7

    June 2018 · Paper 3

    The whole paper ↗
    June 2018 Paper 3, question 03.7: A sample of cyclohexene has been contaminated with cyclohexanol. The cyclohexene can be separated from the cyclohexanol by column chromatography. Silica gel is
  13. Q08.3

    June 2017 · Paper 2

    The whole paper ↗
    June 2017 Paper 2, question 08.3: The dinitrobenzenes shown were investigated by thin layer chromatography (TLC). In an experiment, carried out in a fume cupboard, a concentrated solution of pur
  14. Q08.4

    June 2017 · Paper 2

    The whole paper ↗
    June 2017 Paper 2, question 08.4: State in general terms what determines the distance travelled by a spot in TLC [1 mark
  15. Q08.5

    June 2017 · Paper 2

    The whole paper ↗
    June 2017 Paper 2, question 08.5: To obtain the chromatogram, the TLC plate was held by the edges and placed in the solvent in the beaker in the fume cupboard. The lid was then replaced on the b
  16. Q08.6

    June 2017 · Paper 2

    The whole paper ↗
    June 2017 Paper 2, question 08.6: A second TLC experiment was carried out using 1,2-dinitrobenzene and 1,4-dinitrobenzene. An identical plate to that in Question 8.3 was used under the same cond
  17. Q08.7

    June 2017 · Paper 2

    The whole paper ↗
    June 2017 Paper 2, question 08.7: A third TLC experiment was carried out using 1,2-dinitrobenzene. An identica plate to that in Question 8.3 was used under the same conditions, but the solvent u
  18. Q03.1

    Specimen · Paper 3

    The whole paper ↗
    Specimen Paper 3, question 03.1: 9
  19. Q03.2

    Specimen · Paper 3

    The whole paper ↗
    Specimen Paper 3, question 03.2: Outline the steps needed to locate the positions of the amino acids on the TLC plate and to determine their Rf values. [4 marks] 0 3 . 2
  20. Q03.3

    Specimen · Paper 3

    The whole paper ↗
    Specimen Paper 3, question 03.3: 0
All 20 questions on Chromatography, as a list
  1. June 2024, Paper 3, Q34 (1 mark): The diagram shows a gas chromatogram for a sample containing four isomers with the molecular formula C6H12O2 The carrier gas is nitrogen and the stationary phas
  2. June 2023, Paper 3, Q03.4 (3 marks): Figure 3 shows the apparatus used for the TLC. Figure 3 There is a piece of the apparatus missing from Figure 3. This omission will result in an inaccurate chro
  3. June 2023, Paper 3, Q03.5 (1 mark): State why the amino acids separate on the TLC plate. [1 mark]
  4. June 2023, Paper 3, Q03.6 (2 marks): Name a suitable developing agent. State why the developing agent is needed. [2 marks] Name Why needed
  5. June 2023, Paper 3, Q03.7 (1 mark): Determine the Rf value for Tyr. [1 mark] Rf
  6. November 2021, Paper 3, Q02.1 (2 marks): A student uses thin-layer chromatography (TLC) to identify these amino acids. The student identifies two of the amino acids as alanine and serine. Use Figure 3
  7. November 2021, Paper 3, Q02.2 (1 mark): The amino acids cannot be seen as they move during the experiment. State how the amino acids can be made visible at the end of the experiment. [1 mark]
  8. November 2021, Paper 3, Q02.3 (1 mark): State why each amino acid has a different Rf value. [1 mark]
  9. June 2019, Paper 2, Q09.2 (1 mark): Suggest how the positions of the amino acids on the TLC plate were located. [1 mark]
  10. June 2019, Paper 2, Q09.3 (1 mark): Deduce the minimum number of amino acids present in the original mixture. [1 mark]
  11. June 2019, Paper 2, Q09.4 (1 mark): Suggest why it was necessary to use two different solvents. [1 mark]
  12. June 2018, Paper 3, Q03.7 (2 marks): A sample of cyclohexene has been contaminated with cyclohexanol. The cyclohexene can be separated from the cyclohexanol by column chromatography. Silica gel is
  13. June 2017, Paper 2, Q08.3 (1 mark): The dinitrobenzenes shown were investigated by thin layer chromatography (TLC). In an experiment, carried out in a fume cupboard, a concentrated solution of pur
  14. June 2017, Paper 2, Q08.4 (1 mark): State in general terms what determines the distance travelled by a spot in TLC [1 mark
  15. June 2017, Paper 2, Q08.5 (1 mark): To obtain the chromatogram, the TLC plate was held by the edges and placed in the solvent in the beaker in the fume cupboard. The lid was then replaced on the b
  16. June 2017, Paper 2, Q08.6 (2 marks): A second TLC experiment was carried out using 1,2-dinitrobenzene and 1,4-dinitrobenzene. An identical plate to that in Question 8.3 was used under the same cond
  17. June 2017, Paper 2, Q08.7 (2 marks): A third TLC experiment was carried out using 1,2-dinitrobenzene. An identica plate to that in Question 8.3 was used under the same conditions, but the solvent u
  18. Specimen, Paper 3, Q03.1 (4 marks): 9
  19. Specimen, Paper 3, Q03.2 (4 marks): Outline the steps needed to locate the positions of the amino acids on the TLC plate and to determine their Rf values. [4 marks] 0 3 . 2
  20. Specimen, Paper 3, Q03.3 (2 marks): 0

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