plumiCulturing microorganisms aseptically: Edexcel International A Level Biology questions
Edexcel International A Level Biology past-paper questions on 6.1.1 Culturing microorganisms aseptically, 2019–2025, with mark schemes and examiner’s reports where available.
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Q7(a)(i)
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All 13 questions on Culturing microorganisms aseptically, as a list
- June 2025, Unit 4, Q7(a)(i) (3 marks): Describe how to inoculate the liquid broth using the colonies growing on nutrient agar. (3)
- June 2025, Unit 4, Q7(a)(ii) (2 marks): Suggest why these liquid cultures were shaken in an incubator. (2)
- June 2024, Unit 4, Q2(a)(i) (2 marks): Explain the importance of using aseptic techniques to culture bacteria. (2)
- June 2024, Unit 4, Q2(a)(ii) (2 marks): Explain one aseptic technique that should be used when culturing bacteria. (2)
- January 2023, Unit 4, Q1(a) (2 marks): State two precautions that should be taken to ensure that only one type of bacteria is grown in this culture. (2)
- October 2022, Unit 4, Q1(b)(i) (2 marks): Explain the importance of using an aseptic technique. (2)
- October 2022, Unit 4, Q1(b)(ii) (2 marks): Explain two aseptic techniques that should be used in dilution plating. (2)
- June 2022, Unit 4, Q2(b) (2 marks): Explain how the Petri dish should have been sealed after the mixture was spread onto the agar. (2)
- October 2021, Unit 4, Q5(a)(i) (1 mark): State the meaning of the term aseptic technique. (1)
- October 2021, Unit 4, Q5(a)(ii) (2 marks): Describe two aseptic techniques that could be used in this investigation. (2)
- October 2021, Unit 4, Q5(a)(iii) (2 marks): Explain why using aseptic technique in this investigation is important. (2)
- October 2021, Unit 4, Q5(b) (2 marks): Explain why the microdilution plate had to be incubated at an appropriate temperature for E. coli for 72 hours. (2)
- October 2021, Unit 6, Q4(b) (8 marks): Devise a detailed method, including how you would control and monitor important variables. (8)
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