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Photosynthesis: AQA A‑Level Biology questions

Every AQA A-Level Biology exam question on 3.5.1 Photosynthesis, 2016–2025, with its mark scheme and examiner’s report.

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1,383 questions

  1. Q09.3

    June 2025 · Paper 1

    The whole paper ↗
    June 2025 Paper 1, question 09.3: Use the images in Figure 12 to predict how photosynthesis could be affected in the mutant plants. Justify your answer. [2 marks]
  2. Q09.4

    June 2025 · Paper 1

    The whole paper ↗
    June 2025 Paper 1, question 09.4: Scientists investigated the rate of photosynthesis in healthy plants and in mutant plants. Table 6 shows the scientists’ results. Chlorophyll a and chlorophyll
  3. Q03.1

    June 2024 · Paper 2

    The whole paper ↗
    June 2024 Paper 2, question 03.1: A biologist investigated photosynthesis. They: • measured the percentage of light absorbed by a plant when it was exposed to different wavelengths • measured th
  4. Q03.2

    June 2024 · Paper 2

    The whole paper ↗
    June 2024 Paper 2, question 03.2: Give three environmental factors that should be controlled when measuring the rate of photosynthesis in this investigation. Do not include features of the plant
  5. Q03.3

    June 2024 · Paper 2

    The whole paper ↗
    June 2024 Paper 2, question 03.3: Describe how the products of the light-dependent reaction are used in the light-independent reaction to produce triose phosphate. Do not include the role of rib
  6. Q01.1

    June 2023 · Paper 2

    The whole paper ↗
    June 2023 Paper 2, question 01.1: A student used chromatography to separate the different photosynthetic pigments in a chlorophyll solution. She had the following materials. Chromatography paper
  7. Q01.2

    June 2023 · Paper 2

    The whole paper ↗
    June 2023 Paper 2, question 01.2: Using solvent A, the student separated five pigments. She then repeated her method using a different solvent, B. Using solvent B, she separated six pigments. Ex
  8. Q01.2

    June 2022 · Paper 2

    The whole paper ↗
    June 2022 Paper 2, question 01.2: Describe the light-independent reaction of photosynthesis. [6 marks]
  9. Q05.1

    June 2022 · Paper 3

    The whole paper ↗
    June 2022 Paper 3, question 05.1: Calculate the total uptake of carbon dioxide between 11.40 and 13.15 hours in trees exposed to full sun in a forest that is 12 000 m2 in area. Give your answer
  10. Q05.2

    June 2022 · Paper 3

    The whole paper ↗
    June 2022 Paper 3, question 05.2: Figure 8 shows there is a small difference in the mean uptake of carbon dioxide between 08.30 and 09.40 hours by trees in full sun and by trees in the shade. Wh
  11. Q05.3

    June 2022 · Paper 3

    The whole paper ↗
    June 2022 Paper 3, question 05.3: In this species of tree, very high light intensities can inhibit the release of electrons from chlorophyll. Suggest how this could explain the results shown in
  12. Q03.4

    November 2020 · Paper 2

    The whole paper ↗
    November 2020 Paper 2, question 03.4: The light absorbed by chlorophyll is used in the light-dependent reaction. Name the two products of the light-dependent reaction that are required for the light
  13. Q09.3

    November 2020 · AS Paper 1

    The whole paper ↗
    November 2020 AS Paper 1, question 09.3: Suggest and explain one advantage and one disadvantage of increased stomatal density on the growth of tobacco plant leaves (lines 8–9). [4 marks] Advantage Disa
  14. Q07.1

    June 2019 · Paper 2

    The whole paper ↗
    June 2019 Paper 2, question 07.1: In photosynthesis, which chemicals are needed for the light-dependent reaction? Tick () one box. [1 mark]
  15. Q07.2

    June 2019 · Paper 2

    The whole paper ↗
    June 2019 Paper 2, question 07.2: Describe what happens during photoionisation in the light-dependent reaction. [2 marks]
  16. Q07.3

    June 2019 · Paper 2

    The whole paper ↗
    June 2019 Paper 2, question 07.3: Explain why the student marked the origin using a pencil rather than using ink. [1 mark]
  17. Q07.4

    June 2019 · Paper 2

    The whole paper ↗
    June 2019 Paper 2, question 07.4: Describe the method the student used to separate the pigments after the solution of pigments had been applied to the origin. [2 marks]
  18. Q07.5

    June 2019 · Paper 2

    The whole paper ↗
    June 2019 Paper 2, question 07.5: Calculating the Rf values of the pigments can help to identify each pigment. An Rf value compares the distance the pigment has moved from the origin with the di
  19. Q07.6

    June 2019 · Paper 2

    The whole paper ↗
    June 2019 Paper 2, question 07.6: The pigments in leaves are different colours. Suggest and explain the advantage of having different coloured pigments in leaves. [1 mark]
  20. Q01.1

    June 2018 · Paper 2

    The whole paper ↗
    June 2018 Paper 2, question 01.1: Heat stress decreases the light-dependent reaction of photosynthesis. Explain why this leads to a decrease in the light-independent reaction. [2 marks]
All 31 questions on Photosynthesis, as a list
  1. June 2025, Paper 1, Q09.3 (2 marks): Use the images in Figure 12 to predict how photosynthesis could be affected in the mutant plants. Justify your answer. [2 marks]
  2. June 2025, Paper 1, Q09.4 (3 marks): Scientists investigated the rate of photosynthesis in healthy plants and in mutant plants. Table 6 shows the scientists’ results. Chlorophyll a and chlorophyll
  3. June 2024, Paper 2, Q03.1 (2 marks): A biologist investigated photosynthesis. They: • measured the percentage of light absorbed by a plant when it was exposed to different wavelengths • measured th
  4. June 2024, Paper 2, Q03.2 (2 marks): Give three environmental factors that should be controlled when measuring the rate of photosynthesis in this investigation. Do not include features of the plant
  5. June 2024, Paper 2, Q03.3 (3 marks): Describe how the products of the light-dependent reaction are used in the light-independent reaction to produce triose phosphate. Do not include the role of rib
  6. June 2023, Paper 2, Q01.1 (4 marks): A student used chromatography to separate the different photosynthetic pigments in a chlorophyll solution. She had the following materials. Chromatography paper
  7. June 2023, Paper 2, Q01.2 (2 marks): Using solvent A, the student separated five pigments. She then repeated her method using a different solvent, B. Using solvent B, she separated six pigments. Ex
  8. June 2022, Paper 2, Q01.2 (6 marks): Describe the light-independent reaction of photosynthesis. [6 marks]
  9. June 2022, Paper 3, Q05.1 (3 marks): Calculate the total uptake of carbon dioxide between 11.40 and 13.15 hours in trees exposed to full sun in a forest that is 12 000 m2 in area. Give your answer
  10. June 2022, Paper 3, Q05.2 (2 marks): Figure 8 shows there is a small difference in the mean uptake of carbon dioxide between 08.30 and 09.40 hours by trees in full sun and by trees in the shade. Wh
  11. June 2022, Paper 3, Q05.3 (4 marks): In this species of tree, very high light intensities can inhibit the release of electrons from chlorophyll. Suggest how this could explain the results shown in
  12. November 2020, Paper 2, Q03.4 (2 marks): The light absorbed by chlorophyll is used in the light-dependent reaction. Name the two products of the light-dependent reaction that are required for the light
  13. November 2020, AS Paper 1, Q09.3 (4 marks): Suggest and explain one advantage and one disadvantage of increased stomatal density on the growth of tobacco plant leaves (lines 8–9). [4 marks] Advantage Disa
  14. June 2019, Paper 2, Q07.1 (1 mark): In photosynthesis, which chemicals are needed for the light-dependent reaction? Tick () one box. [1 mark]
  15. June 2019, Paper 2, Q07.2 (2 marks): Describe what happens during photoionisation in the light-dependent reaction. [2 marks]
  16. June 2019, Paper 2, Q07.3 (1 mark): Explain why the student marked the origin using a pencil rather than using ink. [1 mark]
  17. June 2019, Paper 2, Q07.4 (2 marks): Describe the method the student used to separate the pigments after the solution of pigments had been applied to the origin. [2 marks]
  18. June 2019, Paper 2, Q07.5 (1 mark): Calculating the Rf values of the pigments can help to identify each pigment. An Rf value compares the distance the pigment has moved from the origin with the di
  19. June 2019, Paper 2, Q07.6 (1 mark): The pigments in leaves are different colours. Suggest and explain the advantage of having different coloured pigments in leaves. [1 mark]
  20. June 2018, Paper 2, Q01.1 (2 marks): Heat stress decreases the light-dependent reaction of photosynthesis. Explain why this leads to a decrease in the light-independent reaction. [2 marks]
  21. June 2018, Paper 2, Q01.2 (2 marks): Another effect of heat stress is a decrease in the activity of the enzyme rubisco. A decrease in the activity of an enzyme means that the rate of the reaction i
  22. June 2018, Paper 2, Q01.3 (1 mark): Where precisely is rubisco found in a cell? [1 mark] Scientists investigated the effect of temperature on the activity of two enzymes isolated from the leaf cel
  23. June 2018, Paper 2, Q01.4 (4 marks): The scientists concluded that heat stress reduces the activity of rubisco in plant leaves by affecting rubisco activase. Use all the information to evaluate the
  24. June 2017, Paper 2, Q04.1 (2 marks): The solution that the student used to produce the chloroplast suspension had the same water potential as the chloroplasts. Explain why it was important that the
  25. June 2017, Paper 2, Q04.2 (2 marks): Explain why the student set up Tube 1. [2 marks]
  26. June 2017, Paper 2, Q04.3 (2 marks): Explain the results in Tube 3. [2 marks]
  27. June 2017, Paper 2, Q04.4 (1 mark): The student evaluated the effectiveness of different chemicals as weed-killers by assessing their ability to prevent the decolourisation of DCPIP in chloroplast
  28. June 2017, Paper 2, Q04.5 (2 marks): Explain how chemicals which inhibit the decolourisation of DCPIP could slow the growth of weeds. [2 marks]
  29. Specimen, Paper 2, Q08.1 (2 marks): 9
  30. Specimen, Paper 2, Q08.2 (2 marks): Difference
  31. Specimen, Paper 2, Q08.3 (4 marks): [ ] 0

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