Skip to content
Try on iPad

Using gene sequencing: Edexcel A‑Level Biology B questions

Every Edexcel A-Level Biology B exam question on 7.1 Using gene sequencing, 2016–2025, with its mark scheme and examiner’s report.

Find questions1,826
Calculations

1,826 questions

  1. Q9(b)

    June 2025 · Paper 1

    4 marks7.1 Using gene sequencing
    The whole paper ↗
    June 2025 Paper 1, question 9(b): Aptamers can be amplified using PCR. To amplify an RNA aptamer, reverse transcription-PCR (RT-PCR) must be used. Describe how RT-PCR can be used to amplify RNA

  2. Q6(c)

    June 2024 · Paper 1

    4 marks7.1 Using gene sequencing
    The whole paper ↗
    June 2024 Paper 1, question 6(c): Gel electrophoresis can be used to analyse the DNA of a person. The diagram shows some of the bands produced when the DNA of a family was analysed using gel ele

  3. Q1(a)

    November 2021 · Paper 1

    2 marks7.1 Using gene sequencing
    The whole paper ↗
    November 2021 Paper 1, question 1(a): Explain why PCR is used before gel electrophoresis to produce these DNA profiles. (2)

  4. Q1(b)

    November 2021 · Paper 1

    2 marks7.1 Using gene sequencing
    The whole paper ↗
    November 2021 Paper 1, question 1(b): Explain why gel electrophoresis produces individual DNA profiles. (2)

  5. Q1(c)

    November 2021 · Paper 1

    1 mark7.1 Using gene sequencing
    The whole paper ↗
    November 2021 Paper 1, question 1(c): Which of the children have both of these adults as their parents? (1) A child 1 and child 2 B child 1 and child 3 C child 2 and child 3 D child 2 and child 4

  6. Q4(d)(i)

    October 2020 · Paper 3

    1 mark7.1 Using gene sequencing
    The whole paper ↗
    October 2020 Paper 3, question 4(d)(i): State the role of a restriction endonuclease. (1)

  7. Q4(d)(ii)

    October 2020 · Paper 3

    3 marks7.1 Using gene sequencing
    The whole paper ↗
    October 2020 Paper 3, question 4(d)(ii): Describe the process of PCR. (3)

  8. Q1(b)(i)

    June 2019 · Paper 1

    2 marks7.1 Using gene sequencing
    The whole paper ↗
    June 2019 Paper 1, question 1(b)(i): Explain why fragments of DNA can be separated by gel electrophoresis. (2)

  9. Q1(b)(ii)

    June 2019 · Paper 1

    1 mark7.1 Using gene sequencing
    The whole paper ↗
    June 2019 Paper 1, question 1(b)(ii): The graph shows the relationship between the distance moved in a gel and the fragment size. 60 40 20 0 0 1 2 3 Distance moved / cm Determine the size of fragmen
All 9 questions on Using gene sequencing, as a list
  1. June 2025, Paper 1, Q9(b) (4 marks): Aptamers can be amplified using PCR. To amplify an RNA aptamer, reverse transcription-PCR (RT-PCR) must be used. Describe how RT-PCR can be used to amplify RNA
  2. June 2024, Paper 1, Q6(c) (4 marks): Gel electrophoresis can be used to analyse the DNA of a person. The diagram shows some of the bands produced when the DNA of a family was analysed using gel ele
  3. November 2021, Paper 1, Q1(a) (2 marks): Explain why PCR is used before gel electrophoresis to produce these DNA profiles. (2)
  4. November 2021, Paper 1, Q1(b) (2 marks): Explain why gel electrophoresis produces individual DNA profiles. (2)
  5. November 2021, Paper 1, Q1(c) (1 mark): Which of the children have both of these adults as their parents? (1) A child 1 and child 2 B child 1 and child 3 C child 2 and child 3 D child 2 and child 4
  6. October 2020, Paper 3, Q4(d)(i) (1 mark): State the role of a restriction endonuclease. (1)
  7. October 2020, Paper 3, Q4(d)(ii) (3 marks): Describe the process of PCR. (3)
  8. June 2019, Paper 1, Q1(b)(i) (2 marks): Explain why fragments of DNA can be separated by gel electrophoresis. (2)
  9. June 2019, Paper 1, Q1(b)(ii) (1 mark): The graph shows the relationship between the distance moved in a gel and the fragment size. 60 40 20 0 0 1 2 3 Distance moved / cm Determine the size of fragmen

Questions, mark schemes and examiner’s reports are © Pearson Edexcel, from their published past papers. Plumi isn’t affiliated with any exam board. Want whole papers? Every Edexcel A-Level Biology B paper.

Rather write your answers by hand on iPad? Plumi marks them against the same mark schemes.