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Mode of action of enzymes: CAIE A‑Level Biology questions

Every CAIE A-Level Biology exam question on 3.1 Mode of action of enzymes, June 2024 and the specimen papers, with its mark scheme and examiner’s report.

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240 questions

  1. Q1(a)(i)

    Specimen · Paper 5

    The whole paper ↗
    Specimen Paper 5, question 1(a)(i): The student investigated the effect of these two enzymes on the hydrolysis of this protein by incubating the protein separately with each of the enzymes. •• Eac
  2. Q1(a)(ii)

    Specimen · Paper 5

    The whole paper ↗
    Specimen Paper 5, question 1(a)(ii): Identify three variables, other than the chromatography solvent and the specific dye, that the student standardised in this investigation. [1]
  3. Q1(a)(iii)

    Specimen · Paper 5

    The whole paper ↗
    Specimen Paper 5, question 1(a)(iii): Describe how the student could have standardised two of the variables you described in (a)(ii). [2]
  4. Q1(a)(iv)

    Specimen · Paper 5

    The whole paper ↗
    Specimen Paper 5, question 1(a)(iv): Suggest how the student could have determined when hydrolysis of the protein was complete. [1]
  5. Q1(b)

    Specimen · Paper 5

    The whole paper ↗
    Specimen Paper 5, question 1(b): Describe how the student could prepare and use chromatograms to compare the products of hydrolysis of the protein by the two different proteases. Your method sh
  6. Q1(c)

    Specimen · Paper 5

    The whole paper ↗
    Specimen Paper 5, question 1(c): Fig. 1.2 shows the chromatograms produced when complete hydrolysis of the protein by each protease had occurred. Fig. 1.2 also shows the time taken for complete
  7. Q14

    Specimen · Paper 1

    The whole paper ↗
    Specimen Paper 1, question 14: What determines the specificity of an enzyme? 1 the covalent and other bonding between R groups of the polypeptide 2 the optimum pH of the enzyme 3 the covalent
  8. Q3(c)

    Specimen · Paper 2

    The whole paper ↗
    Specimen Paper 2, question 3(c): When developing an enzyme-catalysed reaction for use in industry, the progress of the reaction is studied. Outline how the progress of an enzyme-catalysed react
All 8 questions on Mode of action of enzymes, as a list
  1. Specimen, Paper 5, Q1(a)(i) (1 mark): The student investigated the effect of these two enzymes on the hydrolysis of this protein by incubating the protein separately with each of the enzymes. •• Eac
  2. Specimen, Paper 5, Q1(a)(ii) (1 mark): Identify three variables, other than the chromatography solvent and the specific dye, that the student standardised in this investigation. [1]
  3. Specimen, Paper 5, Q1(a)(iii) (2 marks): Describe how the student could have standardised two of the variables you described in (a)(ii). [2]
  4. Specimen, Paper 5, Q1(a)(iv) (1 mark): Suggest how the student could have determined when hydrolysis of the protein was complete. [1]
  5. Specimen, Paper 5, Q1(b) (8 marks): Describe how the student could prepare and use chromatograms to compare the products of hydrolysis of the protein by the two different proteases. Your method sh
  6. Specimen, Paper 5, Q1(c) (3 marks): Fig. 1.2 shows the chromatograms produced when complete hydrolysis of the protein by each protease had occurred. Fig. 1.2 also shows the time taken for complete
  7. Specimen, Paper 1, Q14 (1 mark): What determines the specificity of an enzyme? 1 the covalent and other bonding between R groups of the polypeptide 2 the optimum pH of the enzyme 3 the covalent
  8. Specimen, Paper 2, Q3(c) (3 marks): When developing an enzyme-catalysed reaction for use in industry, the progress of the reaction is studied. Outline how the progress of an enzyme-catalysed react

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