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Light-independent reactions (Calvin cycle): Edexcel International A Level Biology questions

Edexcel International A Level Biology past-paper questions on 5.1.3 Light-independent reactions (Calvin cycle), 2019–2025, with mark schemes and examiner’s reports where available.

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  1. The start of question 6

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  2. Q4(b)(i)

    January 2025 · Unit 4

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  3. Q4(b)(ii)

    January 2025 · Unit 4

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  4. Q4(b)(iii)

    January 2025 · Unit 4

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  5. The start of question 9

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  6. Q7(a)

    June 2024 · Unit 4

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  7. The start of question 7

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  8. Q2(a)(iii)

    January 2023 · Unit 4

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  9. Q4(b)

    January 2023 · Unit 4

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  10. Q9(b)

    October 2022 · Unit 4

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  11. Q2(b)(ii)

    January 2022 · Unit 4

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  13. Q6(a)(iii)

    January 2022 · Unit 4

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  14. The start of question 1

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  15. The start of question 7

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  16. Q5(b)(i)

    January 2021 · Unit 4

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  17. Q3(b)(ii)

    October 2020 · Unit 4

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  18. Q7(b)(i)

    January 2020 · Unit 4

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  19. Q7(b)(ii)

    January 2020 · Unit 4

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All 19 questions on Light-independent reactions (Calvin cycle), as a list
  1. June 2025, Unit 4, Q6(b)(iii) (4 marks): Describe the roles of the proteins: • RNA polymerase • ribosomal protein • RUBISCO • ATP synthetase. (4)
  2. January 2025, Unit 4, Q4(b)(i) (1 mark): Which describes how ATP is generated in the light‑dependent reactions? (1) A by hydrolysis of ADP releasing energy B by hydrolysis of ADP requiring energy C by
  3. January 2025, Unit 4, Q4(b)(ii) (2 marks): When one molecule of glucose is made in the light‑independent reactions, the ratio of ATP used to reduced NADP used is 1.5 : 1. Show how this ratio can be calcu
  4. January 2025, Unit 4, Q4(b)(iii) (2 marks): Explain why only a small proportion of the GALP produced is used to make the glucose. (2)
  5. October 2024, Unit 4, Q9(b)(iv) (3 marks): Plants treated with blue light had an increase in photosynthesis caused by an increase in the transcription of some genes in the leaves. Suggest two genes whose
  6. June 2024, Unit 4, Q7(a) (3 marks): Describe how carbon becomes incorporated into plant biomass. (3)
  7. June 2024, Unit 4, Q7(b)(i) (2 marks): Explain why air temperature affects the CO2 fertilisation effect. (2)
  8. January 2023, Unit 4, Q2(a)(iii) (1 mark): Where do the light‑independent reactions take place? (1) A Q B S C T D U
  9. January 2023, Unit 4, Q4(b) (4 marks): Rubber is synthesised by specialised cells in the trunks of rubber trees. The diagram shows the structure of one molecule that these cells use to synthesise rub
  10. October 2022, Unit 4, Q9(b) (4 marks): Describe how GALP, produced in the leaves, becomes incorporated into biomass in the roots. (4)
  11. January 2022, Unit 4, Q2(b)(ii) (2 marks): Products of the light‑independent reactions are used to synthesise amino acids. Describe how plants synthesise amino acids. (2)
  12. January 2022, Unit 4, Q6(a)(i) (1 mark): Where in the chloroplast is RUBISCO active? (1) A P B Q C R D S
  13. January 2022, Unit 4, Q6(a)(iii) (1 mark): Where is GP formed? (1) A P B Q C R D S
  14. October 2021, Unit 4, Q1(a)(ii) (1 mark): Which structure contains GALP? (1) A Q B R C U D V
  15. June 2021, Unit 4, Q7(b)(ii) (4 marks): Explain how the products of the light-independent reactions become NPP below the ground. (4)
  16. January 2021, Unit 4, Q5(b)(i) (1 mark): Which row of the table shows the products of the light-dependent reactions that are used in the light-independent reactions? (1) ATP produced by NADP A cyclic p
  17. October 2020, Unit 4, Q3(b)(ii) (1 mark): Where in the chloroplasts are these bonds formed? (1) A cytoplasm B matrix C stroma D thylakoid membrane
  18. January 2020, Unit 4, Q7(b)(i) (3 marks): Describe how GALP, produced in chloroplasts, is converted into protein. (3)
  19. January 2020, Unit 4, Q7(b)(ii) (6 marks): These seaweeds are considered to be a healthy, environmentally-friendly and economic food source. Comment on this statement. Use the information in the table an

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